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forensic toxicology - analytical techniques - HPLC - Statistics
General Stats
- This quiz has been taken 3 times
- The average score is 11 of 43
Answer Stats
| Hint | Answer | % Correct |
|---|---|---|
| either acetonitrile or methanol (non-polar-ish) | 100%
| |
| what is HPLC? | high performance liquid chromatography | 100%
|
| what are the two phases in chromatographic techniques? | the mobile phase | 100%
|
| the stationary phase | 100%
| |
| what is the mobile phase typically made of in HPLC? | water (polar) | 100%
|
| a column | 67%
| |
| column | have stationary phase beads to generate pressure | 67%
|
| pump | split the appropriate gradient (produce the right ratio of solvents used) | 67%
|
| a detector | 33%
| |
| what are the 4 basic parameters of a HPLC system? | a pump | 33%
|
| what is reversed-phase HPLC? | a type of HPLC that has a non-polar stationary phase & a moderately-polar mobile phase | 33%
|
| what is normal-phase HPLC? | a type of HPLC that has a polar stationary phase & a non-polar mobile phase | 33%
|
| what is the most common reversed-phase HPLC? | C18 (silica with 18-carbon chains attached to the surface) | 33%
|
| what kind of data do they generate? | chromatograms | 33%
|
| what are the most common types of detectors for HPLC? | diode array detectors (DAD), a type of UV/vis detector | 33%
|
| mass spectrometer | 33%
| |
| what is the most common sample preparation technique used for HPLC? | solid phase extraction (SPE) | 33%
|
| how does this separate the components? | the components will separate based on the interaction with the sorbent material | 33%
|
| what are chromatographic techniques? | those that involve the separation of mixtures | 33%
|
| how do we analyse these to make this data quantitative? | using peak area & internal standards | 33%
|
| a buffer to aid separation (e.g. formic acid) | 0%
| |
| what is SPE? | a mini form of chromatography used to purify or concentrate the samples before they can be analysed (it also has a mobile & stationary phase) | 0%
|
| an injector | 0%
| |
| column | be porous to increase surface area for separation | 0%
|
| column | contains the stationary phase | 0%
|
| detector | convert the data into useful information | 0%
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| what is this type of HPLC used for? | diagnosis (clinical biochemistry) | 0%
|
| how does this separate the components? | different components of the mixture will separate based on partitioning between the stationary and mobile phases | 0%
|
| injector | draw the sample from a vial into a needle (load position) | 0%
|
| column | have an internal diameter (ID) to determine sensitivity & analyte loading | 0%
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| how does this separate the components? | hydrophilic molecules will be retained by the stationary phase & eluted when the mobile phase gradient changes from non-polar to polar | 0%
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| how does this separate the components? | hydrophobic molecules will be retained by the stationary phase & eluted when the mobile phase gradient changes from polar to non-polar | 0%
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| injector | inject the sample into the flow path of the mobile phase (inject position) | 0%
|
| injector | introduce the sample | 0%
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| how does this help HPLC? | it provides a more pure sample for HPLC to analyse | 0%
|
| what does it entail? | it takes a pressurised liquid (the mobile phase) and passes it through a column that is filled with an absorbent material (the stationary phase) | 0%
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| manufacturing quality control | 0%
| |
| pump | mix the solvents together effectively | 0%
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| pump | move the mobile phase under pressure | 0%
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| pharmaceutical analysis | 0%
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| what do each of these need to do? | pump the solvent | 0%
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| what do they involve? | the mixture being dissolved in fluid | 0%
|
| the mixture being passed through a solid material | 0%
|
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