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forensic toxicology - analytical techniques - mass spectrometry - Statistics
General Stats
- This quiz has been taken 8 times
- The average score is 8 of 51
Answer Stats
| Hint | Answer | % Correct |
|---|---|---|
| what state of matter does the sample have to be in for mass spectrometry? | gas | 86%
|
| electrospray ionisation (ESI) | 57%
| |
| are they a soft or hard technique? | hard (fragments molecules) | 57%
|
| matrix-assisted laser desorption/ionisation (MALDI) | 57%
| |
| ESI | soft (does not fragment molecules) | 57%
|
| MALDI | soft (does not fragment molecules) | 57%
|
| m/z | 43%
| |
| MALDI | a laser irradiated the sample, causing desorption | 29%
|
| what are the three most common ionisation sources used in mass spectrometry? | electron ionisation (EI) | 29%
|
| what pressure can this take place in? | must be in a vacuum | 29%
|
| signal intensity/abundance | 29%
| |
| the ions must then be detected by a detector | 29%
| |
| what is the mass-to-charge ratio (m/z)? | the mass of an ion/the charge of an ion | 29%
|
| MALDI | the sample is mixed with a matrix plate | 29%
|
| ESI | at atmospheric pressure | 14%
|
| MALDI | at atmospheric pressure | 14%
|
| mass spectrum | 14%
| |
| what are the key characteristics of mass analysers used in mass spectrometry? | resolution: the ability to distinguish two peaks of similar m/z | 14%
|
| what is the y-axis for each? | signal intensity | 14%
|
| MALDI | the analytes are then ionised in the plume of desorbed gases | 14%
|
| what is the most common type of detector used in mass spectrometry? | the electron multiplier (EM) | 14%
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| what is the process of mass spectrometry? | the sample must be ionised in an ion source | 14%
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| the second quadrupole is a collision cell, where ions are fragmented by collision with a gas (e.g. argon) (fragmentation) | 14%
| |
| what is the x-axis for each? | time | 14%
|
| EI | accelerated electrons are produced by a filament | 0%
|
| accuracy: the ratio of the measurement error to the true m/z | 0%
| |
| what is a time-of-flight (TOF) mass analyser? | an instrument that accelerates ions in an electric field | 0%
|
| ESI | applies a high voltage to a liquid to create an aerosol containing ions | 0%
|
| how do soft ionisation techniques affect data analysis? | as they add one proton to the molecule, the m/z will increase by 1 | 0%
|
| how do hard ionisation techniques affect data analysis? | because they fragment the molecule, it will lower the molecular weight reading | 0%
|
| what does a quadrupole mass analyser contain? | four rods that are electrically connected | 0%
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| how do they work? | gas phase molecules interact with electrons to produce ions | 0%
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| what is multiple reaction monitoring (MRM)? | hundreds of pairs of ions being scanned | 0%
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| how does it work? | ions move through the mass analyser at a velocity defined by the m/z | 0%
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| how does it work? | it amplifies the signal of the ions | 0%
|
| linear dynamic range: the concentration range over which signal is linear | 0%
| |
| what are the two types of data generated by mass spectrometry analysis? | mass chromatogram | 0%
|
| mass range: the range of m/z that can be analysed | 0%
| |
| EI | molecules collide with the electrons and are ionised and fragmented | 0%
|
| one electron strikes the plate and produces up to 3 more electrons and so on | 0%
| |
| how does a quadrupole mass analyser function? | oscillating electric fields are applied to the rods | 0%
|
| how does mass relate to time of flight? | smaller ions have a faster time of flight | 0%
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| what does this allow? | specific masses to be transmitted | 0%
|
| what is an extracted ion chromatogram (XIC)? | the chromatogram for one or more specific m/z values | 0%
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| what does varying the voltage applied to the quadrupole allow? | the entire mass range to be scanned continuously | 0%
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| what is each used for? | the first quadrupole is used to select the mass(es) of interest (m/z selection) | 0%
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| what is a total ion chromatogram (TIC)? | the intensity of all masses being detected at every point in the analysis | 0%
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| the ions are then separated in an electromagnetic field known as a mass analyser | 0%
| |
| ESI | the solvent in evaporated (desolvation) using hot nitrogen gas to produce free gas-phase ions | 0%
|
| the third quadrupole is used to scan for specific product masses of the mass of interest (m/z selection of fragments) | 0%
| |
| what does a triple quadrupole (QQQ) mass analyser contain? | three quadrupoles connected in series for tandem MS analysis | 0%
|
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