| Hint | Answer | % Correct |
|---|---|---|
| What process is used to amplify DNA? | PCR | 94%
|
| What stage of this process is heated at 55C? | Annealing | 53%
|
| Which end is the DNA inserted at? (Cathode or Anode) | Cathode | 47%
|
| What stage of this process is heated at 95C? | Denaturing | 35%
|
| What type of polymerase is used for this? | Taq polymerase | 18%
|
| How are these strands cut before being added to the wells of the gel? | Restriction Enzymes | 12%
|
| What is the name of the gel involved in this process | Agarose gel (2%) | 6%
|
| What stage of this process is heated at 72C? | Extension | 6%
|
| What bits of DNA are cut? | Variable repeats / VNTRs / STRs | 6%
|
| Why is this useful? | Cannot bind to next nucleotide | 0%
|
| What is the name of the special backbone used in sanger sequencing | Dideoxyribose | 0%
|
| What might you use gel electrophoresis for? | Forensics / crime scene investigation | 0%
|
| Why? | Human polymerase wouldn't survive at this temperature | 0%
|
| What is special about this sugar? | Lacks 2 oxygens | 0%
|
| Why does longer strands of DNA travel a shorter distance in the gel | More resistance | 0%
|