A Level Biology - Manipulating Genomes

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henrydargan
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Last updated: December 9, 2024
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First submittedDecember 8, 2024
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Hint
Answer
What process is used to amplify DNA?
PCR
What stage of this process is heated at 95C?
Denaturing
What stage of this process is heated at 55C?
Annealing
What stage of this process is heated at 72C?
Extension
What type of polymerase is used for this?
Taq polymerase
Why?
Human polymerase wouldn't survive at this temperature
What might you use gel electrophoresis for?
Forensics / crime scene investigation
What is the name of the gel involved in this process
Agarose gel (2%)
Hint
Answer
Why does longer strands of DNA travel a shorter distance in the gel
More resistance
How are these strands cut before being added to the wells of the gel?
Restriction Enzymes
What bits of DNA are cut?
Variable repeats / VNTRs / STRs
Which end is the DNA inserted at? (Cathode or Anode)
Cathode
What is the name of the special backbone used in sanger sequencing
Dideoxyribose
What is special about this sugar?
Lacks 2 oxygens
Why is this useful?
Cannot bind to next nucleotide
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2 Comments
+1
Level 73
Dec 8, 2024
You misspelled "agarose".
+1
Level 46
Dec 10, 2024
I think I've fixed it now