Stats: Biology A-Level Practicals

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Answer Stats

Hint	Answer	% Correct
add 5cm^3 distilled water to one tube to indicate the ____ of enzyme activity	absence/lack	0%
5. set the colorimeter to the filter with the highest ____ (yellow/orange range)	absorbance	0%
10. immediately take a sample and measure ____. repeat every 2 minutes for 10 minutes	absorbance	0%
5. use colorimetry to determine ____. use the red filter. glucose concentration will be directly proportional to ____	absorbance	0%
3. add 20 drops of ____	acetone	0%
9. focus using the coarse and fine ____ ____	adjustment knob/focussing knob	0%
make sure there are no ____ ____ to interfere with the clarity of the image	air bubbles	0%
1. wipe down surfaces with ____ cleaner	antibacterial	0%
5. use sterile forceps to place a multi-disc ____ ring on the plate	antibiotic	0%
3. use two tape measures to create a set of ____ off which coordinates can be read	axes	0%
2. add 2cm^3 of each of the known samples to separate boiling tubes and add 2cm^3 ____ solution	Benedict's	0%
2. add 5cm^3 yeast and 5cm^3 glucose in ____ solution to three test tubes per temperature	buffered	0%
6. plot a ____ ____ of concentration against absorbance	calibration curve	0%
5. use a ____ ____ to extract some of pigment and plot it onto the centre of the baseline	capillary tube	0%
this enzyme hydrolyses the ____ protein in milk, which, when hydrolysed, decolorises the milk	casein	0%
6. transfer to ____ tubes and ____ at a high speed for 10 minutes to separate chloroplasts into the pellet	centrifuge	0%
6. repeat several times and use the ____ ____ test to determine significance	chi squared	0%
1. set up a ____ ____ to have 4 quadrants: high/low light intensity and high/low humidity	choice chamber	0%
ensure the cover slip does not slip sideways as this could damage the ____	chromosomes	0%
hence the DCPIP accepts less electrons so takes long to turn from blue to ____	colourless	0%
6. record time taken to turn _____	colourless	0%
8. measure the absorbance for each solution. a higher absorbance indicates higher pigment ____	concentration	0%
1. make two ____ samples	control	0%
2. set up a Bunsen burner in the work space to create a ____ current to draw microbes away from the culture	convection	0%
3. use a ____ ____ to cut out six potato chips	cork borer	0%
7. lower the ____ ____ down onto the slide	cover slip	0%
6. zero with a ____ of distilled water	cuvette	0%
3. use wet paper towel to make ____ areas and a drying agent to make dry ones	damp/humid	0%
this experiment should be done in a ____ room	darkened	0%
9. place test tube in the rack 30cm from light source and add ____	DCPIP	0%
as temperature increases, permeability increases because membrane proteins ____	denature	0%
6. place each chip in a ____ test tube and leave for 20 minutes	different	0%
1. make a series of ____ of 1M sucrose solution at 0.0, 0.2, 0.4, 0.6, 0.8 and 1.0M	dilutions	0%
1. create a ____ ____ of glucose from concentrations 0 to 10 mmol dm^-3	dilution series	0%
plot a graph of absorbance against time for each ____ from the light	distance	0%
4. remove from acid, wash sample in cold ____ ____, and remove the tip using a scalpel	distilled water	0%
3. place the cubes in test tubes containing an equal volume each of ____ ____	distilled water	0%
8. set colorimeter to red filter and zero using a cuvette containing chloroplast extract and ____ ____	distilled water	0%
7. remove each potato, pat ____ using a paper towel, and take the final masses	dry	0%
2. take 3 test tubes and measure 5cm^3 milk into each and stand in a water bath at 10 degrees C for 5 minutes to ____	equilibrate	0%
3. place them in the water bath and leave them for 10 minutes to ____	equilibrate	0%
9. compare the ____ values to a database	experimental	0%
7. filter each sample into a cuvette using ____ paper	filter	0%
7. repeat with the urine sample and use the calibration curve to determine its ____ concentration	glucose	0%
2. ____ using a pestle and mortar	grind	0%
add 5cm^3 distilled water to the other to indicate the colour of a completely ____ sample	hydrolysed	0%
5. suspend the beaker in an ____ ____ to keep the sample chilled	ice bath	0%
3. transfer root tip to the HCl and ____ for 5 minutes	incubate	0%
6. lightly tape a lid on, invert, and ____ at 25 degrees C for 48 hours	incubate	0%
6. measure the ____ ____	independent variable	0%
4. use a sterile pipette or ____ ____ to transfer bacteria from the broth to the agar plate	inoculating loop	0%
7. remove supernatant and add pellet to the fresh ____ ____ which should be stored on ice	isolation medium	0%
3. place into a chilled ____ ____	isolation solution	0%
the point at which the line of best fit intercepts the x axis is the point where the solution is ____ to the potato	isotonic	0%
11. repeat for different distances from the lamp to vary the ____ ____	light intensity	0%
5. take the ____ of each chip using a balance	mass	0%
4. add 2cm^3 ____ ____ to the test tubes and start the timer	methylene blue	0%
take 2 flat bottomed tubes and add 5cm^3 ____ ____ to each	milk suspension	0%
the stain makes the chromosomes visible and therefore shows which cells are undergoing ____	mitosis	0%
2. cut a section of leaf and place it in a ____	mortar	0%
4. use a ____ ____ and funnel to filter the sample into a beaker	muslin cloth	0%
3. unscrew the bottle and flame the ____	neck	0%
8. place under a microscope and set the ____ lens to the lowest magnification	objective	0%
ensure the lid is not taped around the entire dish as this prevents ____ entering and promotes growth of harmful anaerobic bacteria	oxygen	0%
2. use dark ____ to block out the light on one half	paper	0%
1. draw a ____ line about 1cm from the bottom of the chromatography paper	pencil	0%
8. calculate ____ change in mass for each chip	percentage	0%
5. record ____ ____ of the chosen species	percentage cover	0%
higher pigment concentration indicates a more ____ membrane	permeable	0%
4. use the ____ to grind up the leaf sample to release the pigments	pestle	0%
at low temperatures, the ____ have little energy and are closely packed causing low permeability	phospholipids	0%
as light intensity decreases, rate of photosynthesis decreases because of slower ____	photoionisation	0%
e.g. for light intensity, use a ____ to take a reading	photometer	0%
2. rinse to clean off any ____ released by cutting	pigment	0%
this creates gaps for the ____ molecules to pass through	pigment	0%
5. leave for 10 minutes and record how many are in each ____	quadrant	0%
4. place the ____ at each of the coordinates, placing the bottom left corner on the coordinate each time	quadrat	0%
2. use a ____ ____ ____ to generate 10 sets of random coordinates	random number generator	0%
8. calculate the ____ value for each separated spot	Rf	0%
4. use a ____ to cut them to identical lengths using a scalpel	ruler	0%
1. choose a 5x5m area to take ____ from	samples	0%
2. cut a small sample of the root tip using a ____	scalpel	0%
1. cut beetroot into 6 identical cubes using a ____	scalpel	0%
5. ____ for 10 seconds and return to water baths	shake	0%
5. place the tip on a microscope ____	slide	0%
therefore, a higher absorbance indicates a ____ rate	slower	0%
6. suspend the paper in the ____ so that the level of liquid lies below the pencil line	solvent	0%
7. leave until the solvent has run up the paper near to the top, at which point remove and mark the ____ ____	solvent front	0%
1. remove ____ from leaf samples	stalks	0%
7. ____ equipment and disinfect work surfaces	sterilise	0%
9. plot a graph of percentage change in mass against ____ concentration	sucrose	0%
ensure the ____ is constant because samples very close to the lamp may have an increase	temperature	0%
5. repeat steps 2 to 4 at ____ of 20, 30, 40 and 50 degrees C	temperatures	0%
2. measure 5cm^3 of each dilution into separate ____ ____	test tubes	0%
4. record ____ taken for the milk samples to become colourless	time	0%
6. find the mean time for the milk to be hydrolysed. rate of reaction = 1/____	time	0%
7. find the mean of the results for each temperature and calculate average rate of respiration as 1/____	time	0%
6. add a few drops of a stain such as ____ ____	toluidine blue	0%
4. use ____ to remove the boiling tubes and leave to cool	tongs	0%
3. add 5cm^3 ____ to each tube simultaneously and start the timer	trypsin	0%
1. heat standard hydrochloric acid solution in a ____ ____ at 60 degrees C	water bath	0%
4. place each test tube in a ____ ____ at 20, 30, 40, 50, 60, 70 and 80 degrees and leave for 20 minutes	water bath	0%
3. place boiling tubes in a ____ ____ at 90C for 4 minutes	water bath	0%
1. set up ____ ____ at a range of temperatures	water baths	0%
4. place 10 ____ in the centre of the choice chamber using a spoon	woodlice	0%
the ____ ____ ____ is where bacteria did not grow	zone of inhibition	0%

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Biology A-Level Practicals - Statistics