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Enumerate the Adhesives
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1
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Mayer’s Egg Albumin
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2
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Dried albumin
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3
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1% gelatin
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4
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Gelatin
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5
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Starch paste
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6
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Plasma
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7
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Poly-L-Lysine
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8
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3-APES
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Adhesives
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Most commonly used because it is very easy to make and relatively inexpensive
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Mayer’s Egg Albumin
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formula of mayer’s egg white
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egg white, glycerin, thymol
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formula of dried albumin
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dried albumin, sodium chloride, distilled water, thymol
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formula of 1% gelatin
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gelatin, distilled water, glycerol, phenol crystals
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how much mold inhibitor if added to the formula of 1% gelatin?
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2g
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formula of gelatin
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1% gelatin, 2% formaldehyde
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formula of starch paste
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powdered starch, distilled water, hcl, thymol crystals
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how much plasma is added to each sterile tubes?
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2 mL
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Used as a section adhesive in immunohistochemistry.
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poly-L-Lysine
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final dilution of poly-L-Lysine
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0.01%
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initial concentration of aqueous detergent solution used for the production of poly-L-Lysine
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0.1%
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Very useful in cytology particularly for cytospin preparations of proteinaceous or bloody material.
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3-APES
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best adhesive agent
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3-APES
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complete name of 3-APES
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3-aminopropyltriethoxysilane
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Aqueous/Water Soluble Mountants
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1
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Water
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2
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Glycerin
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3
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Farrant’s Medium
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4
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Apathy’s Medium
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5
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Brun’s Fluid
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Resinous Mountants
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1
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Canada balsam
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2
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DPX
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3
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XAM
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4
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Clarite
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Refractive Indices
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Water
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N/A
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Glycerin
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1.47
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Farrant’s Medium
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1.43
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Apathy’s Medium
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N/A
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Brun’s Fluid
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N/A
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Canada Balsam
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1.524
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DPX
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1.532
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XAM
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1.52
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Clarite
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1.544
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Permanent Mounting Media
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1
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Canada balsam
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2
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DPX
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3
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Clarite
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4
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Eukitt
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5
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Entellan
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Semi-Permanent and Temporary Mounting Media
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1
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Water
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2
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Glycerin
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3
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Mineral oil
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4
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Glycerin jelly
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5
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Von Apathy’s Gum Syrup Medium
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6
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Farrant’s Medium
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7
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Levulose Syrup
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Other recommended synthetic mounting media
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1
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Permount
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2
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H.S.R.
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3
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Clearmount
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Other name of the mountants
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Farrant’s medium
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Gum syrup
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Permount
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Fisher Scientific
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H.S.R.
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Harleco Synthetic Resin
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Clearmount
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Gurr
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Glycerin
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Glycerol
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Mineral oil
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Liquid paraffin
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Glycerin jelly
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Glycerol gelatin
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Levulose Syrup
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Fructose
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Mounting medium
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RI of glass
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1.518
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Designed to mount water-miscible preparations directly from water in cases where the stain is removed or decolorized with alcohol or xylene.
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Aqueous/Water Soluble Mountants
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Used for preparations that have been dehydrated and cleared in xylene or toluene and are recommended for majority of staining methods.
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Resinous mountants
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Low refractive index; good only for temporary mounting
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Water
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Also used as preservative, has high index of refraction
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Glycerin
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Does not solidify upon storage
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Farrant’s Medium
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Used for Methylene Blue-stained nerve preparations and as general purpose aqueous mountant
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Apathy’s Medium
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Recommended for mounting frozen sections from water or paraffin sections which require dehydration and clearing
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Brun’s fluid
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What tree is Canada balsam extracted from?
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Abus balsamea
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Recommended for whole mounts and for thick sections because it does not shrink much
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Canada balsam
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Miscible with xylene and is quite expensive
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Canada balsam
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Recommended for small tissue sections
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DPX
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Complete name of DPX
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Dibutyphthalate polysterene xylene
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Synthetic resin mixture in xylene in pale yellow or colorless solution
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XAM
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Synthetic resin which is soluble in xylene (usually diluted to 60% with xylene)
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Clarite
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These are resins, either natural or synthetic (soluble in alcohol)
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Permanent Mounting Media
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Semi-permanent and temporary mounting media are used to prevent to use of ______ because it dissolves out essential staining such as fat stains.
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permanent xylol-resin mounting media
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Others
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Process of sealing the margins of the coverslip to prevent escape of fluid or semi-fluid mounts and evaporation of mountant, to immobilize the coverslip, and to prevent sticking of slides upon storage
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Ringing
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Made up of 2 parts paraffin wax mixed with 4-9 parts powdered colophonium resin, heated and filtered
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Kronig cement
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Two mounting media most commonly used today
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Eukitt and Entellan
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The temperature of the hot oven utilized for mounting sections that are not properly dehydrated
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50C
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