Fixation Time

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Last updated: December 8, 2025

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Hint	Answer
Formaldehyde	24 hours
10% Formol saline at 35C	24 hours
10% Formol saline at 20-25C	48 hours
10% NBF	4-24 hours
Phosphate Buffered Formalin	4-24 hours
Formol corrosive	3-24 hours
Formol sublimate	3-24 hours
Formol-Mercuric chloride	3-24 hours
Buffered formaldehyde + Osmium tetroxide for small tissue fragments and needle biopsies	2-4 hours
Buffered formaldehyde + Osmium tetroxide for large tissue fragments <4 mm thick	6-8 hours up to 24 hours
Glutaraldehyde	30 minutes to 2 hours

Hint	Answer
Zenker's fluid	12-24 hours
Zenker-formol/Helly's solution	12-24 hours
Heidenhain's Susa solution	3-12 hours
B-5 fixative	1 hour and 30 minutes to 2 hours
Orth’s fluid	36-72 hours
Bouin’s solution	6-24 hours
Ethyl alcohol	18-24 hours
Carnoy’s fluid	1-3 hours
Newcomer’s fluid at 3C	12-18 hours
Flemming’s solution	24-48 hours
Flemming’s solution without acetic acid	24-48 hours

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