Locations of Blood Groups up to Donor Deferrals

Glycophorin A

Glycophorin B

Band 3

AE1

Erythrocyte acetylcholinesterase

p chromosome X

SLC4A1

carried on ART4 by GPI anchor

AQP1

4th component of C4

Glycophorin C and/or D

CR1

Hematopoietic isoform of CD44 marker

CD147

basigin

CD151

Semaphorin 7A

CD108

AQP3

Chromosome 6

HLA Class 1 on RBC

DAF

CD55

 

 

is an important technical aspect in the performance of an antiglobulin test.

Source of error in AHG: Improper specimen (refrigerated, clotted) may cause in vitro complement attachment

Source of error in AHG: Cell suspension either too weak or too heavy

Source of error in AHG: Inadequate or improper washing of cells

Source of error in AHG: AHG reagent, test serum, or enhancement medium not added

Source of error in AHG: Bacterial contamination of cells or saline used in washing.

Source of error in AHG: AHG reagent nonreactive because of deterioration or neutralization (improper reagent storage)

Source of error in AHG: Using a serum sample for a DAT

Source of error in AHG: Cells with a positive DAT will yield a positive IAT.

 

 

22% albumin mechanism

22% albumin incubation time

LISS mechanism

LISS incubation time

What does LISS contain making it low ionic?

What does LISS contain that reduces zeta potential?

PEG mechanism

PEG incubation time

Most sensitive among all potentiators

Describe Coomb’s check cells

pH of saline used in washing cell-serum mixture before performing AHG in test tube

What does LISS contain making it low ionic?

Error if washing of cell-serum mixture is not done before AHG phase

Simulate how Antibody Screening is done

Advantage of test tube method

Disadvantage of test tube method

Advantage of gel technology and solid phase technology method

Disadvantage of gel technology and solid phase technology method

Centrifugation time of gel technology

Incubation time of gel technology

Principle of gel technology

Gel used in gel technology

Screening and panel cells are suspended in what solution

Gel technology applications

Measure of gel card

No. of microtubes consisting the gel card

4+ gel reaction

3+ gel reaction

2+ gel reaction

1+ gel reaction

Mixed-field gel reaction

Negative gel reaction

Antibody class capable of causing agglutination of saline-suspended RBCs

Patient’s history includes

Reagent used in antibody identification

Destroys the pentameric structure of agglutinating IGM

example of sulfhydryl reagents

example of sulfhydryl reagents

process used to physically remove antibodies from sera

process used to remove antibodies bound to RBCs

harvested antibody-containing fluid

measurement of scrubbing

minimum time of scrubbing the site

For blood collection, most blood centers use an iodine compound such as ____ (1)

For blood collection, most blood centers use an iodine compound such as ____ (2)

Donors who are allergic or sensitive to iodine compounds may use

apply tourniquet or blood pressure cuff about ____ to increase distention of the vein

Is the gauge number of needle often used for bleeding/donation

Ask the donor to open and close the hand every _______ during collection procedure

Mix blood and anticoagulant periodically every _____ during the procedure

The conversion factor ____ is used to convert grams to milliliters

Volume of anticoagulant in blood bag

If the volume collected is in the low volume range (300 to 404 mL in a 450-mL collection or 333 to 449 mL in a 500-mL collection), the unit must be labeled as a “low volume unit,” and ______ cannot be made from this unit as it would not contain adequate levels of coagulation factors.

Before the needle is removed from the donor’s arm, pilot tubes are filled. The pressure is reduced to __________

Blood withdrawn over a 7-10 time period and mixed with anticoagulant

Donations of whole blood where the bleed time exceeded ______ are not suitable for the production of plasma components, platelet products, and cryoprecipitate for direct clinical use

Blood collected must be processed for component preparation within __ of collection.

Units in which platelets will be made must be maintained at room temperature _______ until the platelet concentrate has been prepared;

Diversion of at least the first __ of blood into a special diversion pouch can capture skin debris and has been shown to reduce the proportion of platelet components containing viable bacteria.

Governing body for blood bank inspection; inspects blood banks every year

Blood is regulated both as a ___ and as a ____

Blood is regulated both as a ___ and as a ____

Common anticoagulant for apheresis

Type of donation: genetically different individual of the same species

In autologous donation, what are the minimum tests needed to perform?

In the case of autologous donation in which collecting and transfusing facilities are different, what unit should be tested?

In the case of autologous donation in which collecting and transfusing facilities are different, how frequent should the unit be tested?

Autologous units color

The idea of apheresis was developed by

In apheresis, the donor or patient remains attached to the amount of time for a particular procedure can range from

First product to be collected by apheresis methods

Greek word apheresis is derived from

Type of donation: collected under the same requirements as those for allogeneic donors, except that the unit collected is directed toward a specific patient

Directed blood donation color

 

 

General appearance

General appearance

General appearance

General appearance

General requirement as per DOH

General requirement as per DOH

General requirement as per DOH

Age (AABB)

Age (DOH)

needed document of a 16-17 year old donor

donor age that needs physician consent

Regular donors may be accepted up to ___ yrs old (before ___ birthday) subject to evaluation by a medical officer

Weight in kg

Weight in pounds

Standards mandates a maximum of ___ of blood/kg of donor weight for whole blood collection, inclusive of pilot tubes for testing.

Temperature in celcius

Temperature in fahrenheit

Pulse as per trans

Pulse as per DOH

Pulse of an athletic — not a cause for deferral

Blood pressure systolic as per AABB

Blood pressure diastolic as per AABB

Blood pressure systolic as per DOH

Blood pressure diastolic as per DOH

Hemoglobin as per AABB (Female)

Hemoglobin as per AABB (Male)

Range of Hemoglobin as per DOH (Female)

Range of Hemoglobin as per DOH (Male)

Hematocrit of women

Hematocrit of men

CuSO4 specific gravity should be

Volume of the container to be used in CuSO4 method

Distance between drop of blood and solution should be

Acceptable drop of blood will sink in solution within ______ if Hb concentration is greater than or equal to 12.5 mg/dL

Age

Weight

Hemoglobin

Hematocrit

Frequency

Occurs during the 5 to 6 weeks immediately preceding a scheduled, elective surgical procedure unless the red blood cells and plasma are scheduled to be frozen.

The last blood collection should occur no later than __________ before the scheduled surgery to allow for volume replacement.

Results in the collection of whole blood with the concurrent infusion of crystalloid or colloid solutions, thus maintaining a normal blood volume but decreasing the patient’s hematocrit

Ratio of replacement for crystalloids

Ratio of replacement for colloids

Involves collecting shed blood from the surgical site; processing the blood through an instrument that washes it with saline to remove tissue debris, free hemoglobin, and plasma that may contain activated coagulation factors; concentrating the residual red cells (to a hematocrit of 50% to 60%); and then reinfusing those cells immediately.

In the 3rd method of obtaining autologous blood, how much is the hematocrit of the residual red cells concentrated into before reinfusing the cells?

a drainage tube is placed in the surgical site and postoperative bleeding is salvaged, cleaned and reinfused

Prospective donor is unable to donate blood for a limited period of time.

Prospective donor is unable to donate blood for someone else for an unspecified period of time due to current regulatory requirements.

Prospective donor will never be eligible to donate blood for someone else.

This donor would not be able to donate blood until the current requirement changes.

Chronic cardiopulmonary disease

Tattoo

Received clotting factors

After Hepa B immunoglobulin administration

Chicken pox vaccination

Positive for HTLV

Finasteride (Proscar)

Rape victim

Leukemia, lymphoma, or myeloproliferative disorder

Recipient of human pituitary—derived growth hormone

Received transfusion of blood components, or other human tissues (organ, bone, marrow, or skin graft)

Positive for AIDS

Recipient of bovine insulin

Experimental medication or unlicensed (experimental) vaccine

German measles (rubella) vaccination

Needle stick injury

Renal disease (chronic)

IV drug user

Sexual contact with prostitute, person with AIDS/hepatitis, or hemophiliac, or IV drug user

Recipient of cornea and dura mater (trans)

Recipient of cornea and dura mater (DOH)

Positive for HBsAg

Major operation including dental surgery

Female who had sexual contact with a man who ever had sex with another man

History of babesiosis

Syphilis or gonorrhea

History of Chagas disease

Etretinate (Tegison’ treatment for psoriasis

Healthcare worker exposed to blood or body fluids

Ear or body piercing

Malignant solid tumors

Liver disease (chronic)

Have been in prison, detention or lockup >72 hours

Isotretinoin (Accutane) for acne

Rabies vaccination

Hemophiliacs

Tegison

Childbirth (AABB)

Childbirth (WHO)

Childbirth (DOH)

Is abortion in 1st trimester or 2nd trimester a cause for deferral?

Transfusion during pregnancy

MMR combined (DOH)

MMR combined (book)

2 weeks referral due to vaccination

2 weeks referral due to vaccination

2 weeks referral due to vaccination

2 weeks referral due to vaccination

2 weeks referral due to vaccination

Proscar

Soriatane

Accutane

Propecia

Avodart

Plavix

Feldene

Effient

Brilinta

Ticlid

Zontivity

Aspirin (Book)

Aspirin (DOH)

Piroxicam (Book)

Piroxicam (DOH)

HIV Prevention

PrEP or PEP

Malaria Deferral: Travelers to endemic areas (AABB)

Malaria Deferral: Immigrants, refuges, citizens who resided on endemic areas, and diagnosed/infected with malaria (AABB)

Malaria Deferral: Malaria Infection (DOH)

Malaria Deferral: Travel to area endemic for malaria with or without prophylaxis (stay for < 6 months)

Malaria Deferral: Former resident of malaria endemic area, or stayed in malaria endemic area for 6 months or more

Whole Blood Donation (AABB)

Whole Blood Donation (DOH)

Toxoid/killed/synthetic vaccines