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1. What is Spider-Man’s real name?
2. What newspaper does Peter Parker work for?
3. Who is Spider-Man’s most famous enemy?
4. What radioactive creature bit Peter Parker?
5. What is Spider-Man’s Aunt’s name?
6. What’s the name of Peter Parker’s uncle whose death inspires him to be a hero?
7. Who killed Gwen Stacy in the comics?
8. Which villain uses mechanical tentacles?
9. Which villain runs Oscorp?
10. Which villain is a master of illusions?
11. What futuristic Spider-Man lives in the year 2099?
12. Who is Peter Parker’s best friend in most stories?
13. Which villain hunts Spider-Man for sport?
14. What is Spider-Man’s symbiote black suit originally?
15. Who becomes the Scarlet Spider after Peter Parker?
16. In Spider-Man: No Way Home (2021), which spell causes the multiverse to break open?
17. Which symbiote is the offspring of Venom?
18. Which alternate universe Spider-Man is a pig?
19. Who was Peter Parker’s first publisher boss at the Daily Bugle?
20. What MCU movie was Spider-Man’s first appearance?
The question about the most famous enemy is subjective I'd say
Riot is also an offspring of Venom
The first MCU movie in which Peter Parker appeared was Iron Man 2