separation techniques 2

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Last updated: January 18, 2023

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First submitted	January 18, 2023
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question	Answer
what type of FPLC used a specific buffer pH and a resin with basic side chains ?	ion exchange
when creating a salt gradient in FPLC ion exchange we mix buffer 'a' with buffer 'b' (e.g. 1MNaCl), weak bound proteins will fall off at a lower concentration than target proteins true or false ?	true
FPLC gel filtration is usedinstead of the other FPLC methods when the protein is damaged by ....	salt
in FPLC gel filtration what size proteins falls through the column first ?	large

question	Answer
what blue molecule is commonly used as a marker for large proteins in FPLC gel filtration ?	blue dextran
what pink molecule is commonly used as a marker for small proteins in FPPLC gel filtration?	vitamin b12
what separation technique is best suited to small biological molecules ?	HPLC
what type of HPLC consists of a non polar solvent and a polar stationary phase ?	forward phase