Guess the Organism - Microbiology

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okerrigan
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Last updated: April 4, 2025
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First submittedApril 4, 2025
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1. Large, gray-white colonies with a double zone of beta-hemolysis on anaerobic blood agar. Gram stain reveals large, boxcar-shaped Gram-positive rods.
Clostridium perfringens
Clostridium difficile
Listeria monocytogenes
Bacillus cereus
2. Small, creamy, beta-hemolytic colonies grow on blood agar. The organism is catalase positive, coagulase positive, and Gram stain reveals Gram-positive cocci in clusters.
Micrococcus luteus
Staphylococcus epidermidis
Enterococcus faecalis
Staphylococcus aureus
3. A colony shows swarming motility on blood agar. The organism is oxidase negative and urease positive. It produces hydrogen sulfide on TSI agar.
Proteus mirabilis
Salmonella enteritidis
Escherichia coli
Proteus vulgaris
4. Lactose-fermenting pink colonies grow on MacConkey agar. The organism is indole positive, oxidase negative, and Gram-negative rods are seen on staining.
Escherichia coli
Citrobacter freundii
Klebsiella pneumoniae
Shigella spp.
5. Tiny gray colonies grow on chocolate agar but not on blood agar. Gram-negative coccobacilli are seen on Gram stain. The isolate requires both X and V factors.
Neisseria gonorrhoeae
Moraxella catarrhalis
Haemophilus influenzae
Neisseria meningitidis
6. Alpha-hemolytic colonies appear on blood agar. The isolate is catalase negative, optochin sensitive, and bile soluble. Gram-positive lancet-shaped diplococci are seen.
Streptococcus pneumoniae
Enterococcus gallolyticus
Streptococcus pyogenes
Viridans group streptococci
7. Beta-hemolytic colonies appear on blood agar. Catalase negative, bacitracin sensitive, Gram-positive cocci in chains.
Streptococcus pyogenes
Streptococcus agalactiae
Staphylococcus saprophyticus
Enterococcus faecium
8. Colonies have a mucoid appearance on MacConkey agar. The organism is urease positive, indole negative, oxidase negative.
Klebsiella pneumoniae
Proteus vulgaris
Shigella sonnei
Klebsiella oxytoca
9. A sputum culture yields colonies with a “breadcrumb-like” appearance. The organism is weakly acid-fast and filamentous on Gram stain.
Mycobacterium tuberculosis
Corynebacterium diphtheriae
Actinomyces israelii
Nocardia spp.
10. A patient with HIV presents with meningitis. India ink staining of CSF reveals encapsulated yeast. Colonies grow on Sabouraud Dextrose Agar (SDA) and are urease positive.
Histoplasma capsulatum
Cryptococcus neoformans
Candida albicans
Aspergillus fumigatus
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