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Best fixative for...

Calcium acetate formalin/Lillie's fixative - type Calcium acetate formalin only Alcoholic formalin/Gendre's fixative - type Alcoholic formalin only Zenker-formol/Helly's solution - Zenker-formol Schaudinn's solution/Sublimated alcohol - Schaudinn's solution Regaud's fluid/Moeller's fluid
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Last updated: December 8, 2025
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First submittedDecember 8, 2025
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BEST FIXATIVE FOR NERVOUS TISSUE
Formaldehyde
Traditionally, it is the most commonly used fixative in pathology
10% formol saline
BEST FIXATIVE FOR CENTRAL NERVOUS SYSTEM
10% formol saline
General post-mortem tissues for histochemical examination
10% formol saline
Preserves enzymes and nucleoproteins, and demonstrates fats and lipids
10% formol saline
Ideal for silver impregnation
10% formol saline
Used to preserve phospholipids
Calcium acetate formalin
Replaced formol-saline as the most commonly used fixative in pathology
Calcium acetate formalin
BEST GENERAL TISSUE FIXATIVE
10% NBF
BEST FIXATIVE FOR FROZEN SECTIONS
10% NBF
BEST FIXATIVE FOR IRON-CONTAINING PIGMENTS AND ELASTIC FIBERS
10% NBF
Recommended for surgical, post mortem and research specimens
10% NBF
Prevents precipitation of acid formalin pigments
10% NBF
Recommended for routine post-mortem tissues.
Formol corrosive
It is excellent for silver reticulum methods.
Formol corrosive
Fixes lipids, especially neutral fats and phospholipids.
Formol corrosive
For sputum cytology
Alcoholic formalin
Good fixative for nervous tissue (astrocytes)
Cajol's formol ammonium bromide
Used for the preservation of lipids since most formalin fixatives are inert to lipids.
Baker's formol calcium
Recommended for Histochemistry and electron microscopy
Glutaraldehyde
Most common metallic fixative
Mercuric chloride
The routine fixative of choice for preservation of cell detail in tissue photography
Mercuric chloride
Excellent for trichrome staining
Mercuric chloride
Recommended for renal tissues, fibrin, connective tissues, and muscles
Mercuric chloride
Good general fixative for all kinds of tissue
Zenker's fluid
Recommended for fixing small pieces of liver, spleen, connective tissue fibers and nuclei
Zenker's fluid
Recommended for Trichrome staining
Zenker's fluid
Excellent microanatomic fixative for pituitary gland, bone marrow and blood-containing organs such as the liver and spleen.
Zenker-formol
Recommended for tumor biopsies especially of the skin
Heidenhain's susa solution
Excellent cytologic fixative
Heidenhain's susa solution
Used on wet smears for cytologic examinations.
Schaudinn's solution
Commonly used for bone marrow biopsies
B-5 fixative
Recommended for Chromaffin tissues, Adrenal medulla, Mitochondria
Chromate
Recommended for demonstration of Chromatin, Mitochondria, Mitotic figures, Golgi bodies, RBC’s and colloid-containing tissues
Moeller's fluid
Recommended for study of early degenerative
processes and tissue necrosis
Orth's fluid
Hint
Answer
Excellent for glycogen demonstration
Picric acid fixatives
Can be used for fragmentary biopsies
Picric acid fixatives
Suitable for aniline stains and Trichrome method
Picric acid fixatives
Fixation of embryos and pituitary biopsies
Bouin's solution
Excellent fixative for preserving soft and delicate structures (endometrial curetting’s)
Bouin's solution
Useful in fragmentary biopsies
Bouin's solution
Preferred fixative for Masson’s trichrome staining for collagen, elastic or connective tissue
Bouin's solution
It is recommended for acid mucopolysaccharides
Lead fixatives
It fixes connective tissue mucin
Lead fixatives
Best routine fixative for glycogen
Brasil's Alcoholic Picroformol Fixative
Fixes and precipitates nucleoproteins
Glacial acetic acid
It precipitates chromosomes and chromatin materials.
Glacial acetic acid
Denatures and precipitates proteins
Alcohol fixatives
May be used both as a fixative and dehydrating agent
Alcohol fixatives
Excellent for glycogen preservation
Alcohol fixatives
Used in Wright’s stain as a diluent
100% Methyl alcohol
Excellent for fixing wet and dry smears, blood smears and bone marrow tissues
100% Methyl alcohol
It is used for fixing touch preparations (impression smears)
95% Isopropyl alcohol
It fixes blood, tissue films and smears
Ethyl alcohol
Used for histochemistry especially for enzyme studies
Ethyl alcohol
The most rapid tissue fixative
Carnoy’s fluid
Recommended for fixing chromosome, lymph glands and urgent biopsies
Carnoy’s fluid
It is used to fix brain tissue for rabies diagnosis
Carnoy’s fluid
It is very suitable for curettings (small tissue fragments)
Carnoy’s fluid
Is both a nuclear and histochemical fixative
Newcomer's fluid
Adequately fixes materials for ultrathin sectioning in electron microscopy, since it rapidly fixes small pieces of tissues and aids in their staining
Osmium tetroxide
The most commonly used Chrome-Osmium acetic acid, recommended for nuclear preparation of such sections.
Flemming’s solution
Excellent for nuclear structures such as chromosomes
Flemming’s solution
Permanently fixes fats/lipids
Flemming’s solution
It depresses the staining power of Ehrlich hematoxylin
Flemming’s solution
Recommended for cytoplasmic structures such as mitochondria
Flemming’s Solution without Acetic Acid
Suitable only for small pieces of tissues or one because of its poor penetration
Trichloroacetic acid
Recommended for the study of water diffusible enzymes especially lipases and phosphatases
Acetone
Used in freeze substitution techniques as a solvent for certain metallic salts
Acetone
Also for rapid diagnosis, usually employed for frozen tissue sections and preparations of bacteriologic smears
Heat fixation
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