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Hint
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Answer
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BEST FIXATIVE FOR NERVOUS TISSUE
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Formaldehyde
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Traditionally, it is the most commonly used fixative in pathology
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10% formol saline
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BEST FIXATIVE FOR CENTRAL NERVOUS SYSTEM
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10% formol saline
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General post-mortem tissues for histochemical examination
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10% formol saline
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Preserves enzymes and nucleoproteins, and demonstrates fats and lipids
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10% formol saline
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Ideal for silver impregnation
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10% formol saline
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Used to preserve phospholipids
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Calcium acetate formalin
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Replaced formol-saline as the most commonly used fixative in pathology
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Calcium acetate formalin
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BEST GENERAL TISSUE FIXATIVE
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10% NBF
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BEST FIXATIVE FOR FROZEN SECTIONS
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10% NBF
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BEST FIXATIVE FOR IRON-CONTAINING PIGMENTS AND ELASTIC FIBERS
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10% NBF
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Recommended for surgical, post mortem and research specimens
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10% NBF
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Prevents precipitation of acid formalin pigments
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10% NBF
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Recommended for routine post-mortem tissues.
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Formol corrosive
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It is excellent for silver reticulum methods.
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Formol corrosive
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Fixes lipids, especially neutral fats and phospholipids.
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Formol corrosive
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For sputum cytology
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Alcoholic formalin
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Good fixative for nervous tissue (astrocytes)
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Cajol's formol ammonium bromide
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Used for the preservation of lipids since most formalin fixatives are inert to lipids.
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Baker's formol calcium
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Recommended for Histochemistry and electron microscopy
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Glutaraldehyde
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Most common metallic fixative
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Mercuric chloride
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The routine fixative of choice for preservation of cell detail in tissue photography
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Mercuric chloride
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Excellent for trichrome staining
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Mercuric chloride
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Recommended for renal tissues, fibrin, connective tissues, and muscles
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Mercuric chloride
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Good general fixative for all kinds of tissue
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Zenker's fluid
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Recommended for fixing small pieces of liver, spleen, connective tissue fibers and nuclei
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Zenker's fluid
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Recommended for Trichrome staining
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Zenker's fluid
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Excellent microanatomic fixative for pituitary gland, bone marrow and blood-containing organs such as the liver and spleen.
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Zenker-formol
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Recommended for tumor biopsies especially of the skin
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Heidenhain's susa solution
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Excellent cytologic fixative
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Heidenhain's susa solution
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Used on wet smears for cytologic examinations.
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Schaudinn's solution
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Commonly used for bone marrow biopsies
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B-5 fixative
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Recommended for Chromaffin tissues, Adrenal medulla, Mitochondria
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Chromate
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Recommended for demonstration of Chromatin, Mitochondria, Mitotic figures, Golgi bodies, RBC’s and colloid-containing tissues
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Moeller's fluid
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Recommended for study of early degenerative
processes and tissue necrosis
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Orth's fluid
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Excellent for glycogen demonstration
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Picric acid fixatives
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Can be used for fragmentary biopsies
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Picric acid fixatives
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Suitable for aniline stains and Trichrome method
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Picric acid fixatives
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Fixation of embryos and pituitary biopsies
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Bouin's solution
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Excellent fixative for preserving soft and delicate structures (endometrial curetting’s)
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Bouin's solution
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Useful in fragmentary biopsies
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Bouin's solution
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Preferred fixative for Masson’s trichrome staining for collagen, elastic or connective tissue
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Bouin's solution
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It is recommended for acid mucopolysaccharides
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Lead fixatives
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It fixes connective tissue mucin
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Lead fixatives
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Best routine fixative for glycogen
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Brasil's Alcoholic Picroformol Fixative
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Fixes and precipitates nucleoproteins
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Glacial acetic acid
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It precipitates chromosomes and chromatin materials.
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Glacial acetic acid
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Denatures and precipitates proteins
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Alcohol fixatives
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May be used both as a fixative and dehydrating agent
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Alcohol fixatives
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Excellent for glycogen preservation
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Alcohol fixatives
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Used in Wright’s stain as a diluent
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100% Methyl alcohol
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Excellent for fixing wet and dry smears, blood smears and bone marrow tissues
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100% Methyl alcohol
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It is used for fixing touch preparations (impression smears)
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95% Isopropyl alcohol
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It fixes blood, tissue films and smears
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Ethyl alcohol
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Used for histochemistry especially for enzyme studies
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Ethyl alcohol
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The most rapid tissue fixative
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Carnoy’s fluid
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Recommended for fixing chromosome, lymph glands and urgent biopsies
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Carnoy’s fluid
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It is used to fix brain tissue for rabies diagnosis
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Carnoy’s fluid
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It is very suitable for curettings (small tissue fragments)
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Carnoy’s fluid
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Is both a nuclear and histochemical fixative
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Newcomer's fluid
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Adequately fixes materials for ultrathin sectioning in electron microscopy, since it rapidly fixes small pieces of tissues and aids in their staining
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Osmium tetroxide
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The most commonly used Chrome-Osmium acetic acid, recommended for nuclear preparation of such sections.
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Flemming’s solution
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Excellent for nuclear structures such as chromosomes
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Flemming’s solution
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Permanently fixes fats/lipids
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Flemming’s solution
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It depresses the staining power of Ehrlich hematoxylin
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Flemming’s solution
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Recommended for cytoplasmic structures such as mitochondria
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Flemming’s Solution without Acetic Acid
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Suitable only for small pieces of tissues or one because of its poor penetration
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Trichloroacetic acid
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Recommended for the study of water diffusible enzymes especially lipases and phosphatases
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Acetone
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Used in freeze substitution techniques as a solvent for certain metallic salts
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Acetone
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Also for rapid diagnosis, usually employed for frozen tissue sections and preparations of bacteriologic smears
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Heat fixation
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