Best fixative for... - Statistics

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Hint Answer % Correct
Used in Wright’s stain as a diluent 100% Methyl alcohol
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Excellent for fixing wet and dry smears, blood smears and bone marrow tissues 100% Methyl alcohol
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Traditionally, it is the most commonly used fixative in pathology 10% formol saline
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BEST FIXATIVE FOR CENTRAL NERVOUS SYSTEM 10% formol saline
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General post-mortem tissues for histochemical examination 10% formol saline
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Preserves enzymes and nucleoproteins, and demonstrates fats and lipids 10% formol saline
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Ideal for silver impregnation 10% formol saline
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BEST GENERAL TISSUE FIXATIVE 10% NBF
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BEST FIXATIVE FOR FROZEN SECTIONS 10% NBF
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BEST FIXATIVE FOR IRON-CONTAINING PIGMENTS AND ELASTIC FIBERS 10% NBF
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Recommended for surgical, post mortem and research specimens 10% NBF
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Prevents precipitation of acid formalin pigments 10% NBF
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It is used for fixing touch preparations (impression smears) 95% Isopropyl alcohol
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Recommended for the study of water diffusible enzymes especially lipases and phosphatases Acetone
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Used in freeze substitution techniques as a solvent for certain metallic salts Acetone
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Denatures and precipitates proteins Alcohol fixatives
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May be used both as a fixative and dehydrating agent Alcohol fixatives
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Excellent for glycogen preservation Alcohol fixatives
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For sputum cytology Alcoholic formalin
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Commonly used for bone marrow biopsies B-5 fixative
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Used for the preservation of lipids since most formalin fixatives are inert to lipids. Baker's formol calcium
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Fixation of embryos and pituitary biopsies Bouin's solution
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Excellent fixative for preserving soft and delicate structures (endometrial curetting’s) Bouin's solution
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Useful in fragmentary biopsies Bouin's solution
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Preferred fixative for Masson’s trichrome staining for collagen, elastic or connective tissue Bouin's solution
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Best routine fixative for glycogen Brasil's Alcoholic Picroformol Fixative
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Good fixative for nervous tissue (astrocytes) Cajol's formol ammonium bromide
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Used to preserve phospholipids Calcium acetate formalin
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Replaced formol-saline as the most commonly used fixative in pathology Calcium acetate formalin
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The most rapid tissue fixative Carnoy’s fluid
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Recommended for fixing chromosome, lymph glands and urgent biopsies Carnoy’s fluid
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It is used to fix brain tissue for rabies diagnosis Carnoy’s fluid
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It is very suitable for curettings (small tissue fragments) Carnoy’s fluid
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Recommended for Chromaffin tissues, Adrenal medulla, Mitochondria Chromate
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It fixes blood, tissue films and smears Ethyl alcohol
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Used for histochemistry especially for enzyme studies Ethyl alcohol
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The most commonly used Chrome-Osmium acetic acid, recommended for nuclear preparation of such sections. Flemming’s solution
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Excellent for nuclear structures such as chromosomes Flemming’s solution
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Permanently fixes fats/lipids Flemming’s solution
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It depresses the staining power of Ehrlich hematoxylin Flemming’s solution
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Recommended for cytoplasmic structures such as mitochondria Flemming’s Solution without Acetic Acid
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BEST FIXATIVE FOR NERVOUS TISSUE Formaldehyde
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Recommended for routine post-mortem tissues. Formol corrosive
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It is excellent for silver reticulum methods. Formol corrosive
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Fixes lipids, especially neutral fats and phospholipids. Formol corrosive
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Fixes and precipitates nucleoproteins Glacial acetic acid
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It precipitates chromosomes and chromatin materials. Glacial acetic acid
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Recommended for Histochemistry and electron microscopy Glutaraldehyde
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Also for rapid diagnosis, usually employed for frozen tissue sections and preparations of bacteriologic smears Heat fixation
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Recommended for tumor biopsies especially of the skin Heidenhain's susa solution
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Excellent cytologic fixative Heidenhain's susa solution
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It is recommended for acid mucopolysaccharides Lead fixatives
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It fixes connective tissue mucin Lead fixatives
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Most common metallic fixative Mercuric chloride
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The routine fixative of choice for preservation of cell detail in tissue photography Mercuric chloride
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Excellent for trichrome staining Mercuric chloride
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Recommended for renal tissues, fibrin, connective tissues, and muscles Mercuric chloride
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Recommended for demonstration of Chromatin, Mitochondria, Mitotic figures, Golgi bodies, RBC’s and colloid-containing tissues Moeller's fluid
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Is both a nuclear and histochemical fixative Newcomer's fluid
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Recommended for study of early degenerative processes and tissue necrosis Orth's fluid
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Adequately fixes materials for ultrathin sectioning in electron microscopy, since it rapidly fixes small pieces of tissues and aids in their staining Osmium tetroxide
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Excellent for glycogen demonstration Picric acid fixatives
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Can be used for fragmentary biopsies Picric acid fixatives
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Suitable for aniline stains and Trichrome method Picric acid fixatives
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Used on wet smears for cytologic examinations. Schaudinn's solution
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Suitable only for small pieces of tissues or one because of its poor penetration Trichloroacetic acid
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Excellent microanatomic fixative for pituitary gland, bone marrow and blood-containing organs such as the liver and spleen. Zenker-formol
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Good general fixative for all kinds of tissue Zenker's fluid
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Recommended for fixing small pieces of liver, spleen, connective tissue fibers and nuclei Zenker's fluid
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Recommended for Trichrome staining Zenker's fluid
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