Clearing Agents - Statistics

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General Stats

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Answer Stats

Hint	Answer	% Correct
Usual clearing time of xylene	1/2-2 hours	100%
Clearing time of toluene	1-2 hours	100%
Urgent biopsy clearing time of xylene	15-30 minutes	100%
Clearing time of benzene	15-60 minutes	100%
clearing time of cedarwood oil	2-3 days	100%
clearing time of chloroform	6-24 hours	100%
Recommended for clearing embryos, insects and very delicate specimens since it clears 70% alcohol without excessive tissue shrinkage and hardening	aniline oil	100%
Carcinogenic or may damage bone marrow (Aplastic anemia; If antibiotic: Chloramphenicol)	benzene	100%
Rapid acting, recommended for urgent biopsies and routine purposes	benzene	100%
Toxic on prolonged exposure	CCl4	100%
Properties are very similar to chloroform but it is cheaper	CCl4	100%
It becomes milky on prolonged storage	cedarwood oil	100%
Quality is not always uniform and good and is extremely slow	cedarwood oil	100%
Recommended for CNS tissues and cytological studies (esp. Smooth muscles and skin)	cedarwood oil	100%
Clears both Paraffin and Celloidin sections	cedarwood oil	100%
Very expensive and it requires 2 changes in clearing solution	cedarwood oil	100%
It is the best of the traditional clearing agents for routine use	chloroform	100%
Gives the widest latitude	chloroform	100%
Best for nervous tissue, lymph nodes, granulation tissue, and fetal and other delicate, highly cellular specimens, all of which tend to become distorted and to break up on sectioning if cleared in xylene, toluene, or benzene	chloroform	100%
It does not make tissues transparent and it is toxic to the liver (hepatotoxic) after prolonged inhalation	chloroform	100%
It is slower in action than xylene, but causes less brittleness	chloroform	100%
It is recommended for tough (skin, fibroid and decalcified tissues) and large tissue specimens	chloroform	100%
Not suitable for routine purposes because it is expensive	clove oil	100%
It removes aniline dyes and dissolves Celloidin; Tissues become brittle	clove oil	100%
Causes minimum shrinkage of tissues	clove oil	100%
Its quality is not guaranteed due to its tendency to be adulterated	clove oil	100%
Block size for xylene	<5 mm	100%
These are slow-acting clearing agents that can be used when double embedding techniques are required.	Methyl benzoate	100%
Dehydrates and clears at the same time since it is miscible in both water and paraffin	Tetrahydrofuran	100%
It acts slower than benzene and is expensive	Toluene	100%
Substitute to xylene or benzene	toluene	100%
It is not carcinogenic but highly concentrated emit fumes that are toxic upon prolonged exposure	Toluene	100%
If tissue or section is incompletely dehydrated, it becomes milky	xylene	100%
It causes considerable hardening and shrinkage of tissues; hence, is not suitable for nervous tissues and lymph nodes.	xylene	100%
An excellent and true clearing agent	xylene	100%
The most rapid clearing agent	xylene	100%
Cheap and does not extract out aniline dyes	xylene	100%
Can be used with celloidin sections	xylene	100%
Highly flammable	xylene, benzene	100%
It is miscible with absolute alcohol and paraffin	xylene, toluene, benzene	100%

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