Fixation Time

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yannnnn
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Last updated: December 8, 2025
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First submittedDecember 8, 2025
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Answer
Formaldehyde
24 hours
10% Formol saline at 35C
24 hours
10% Formol saline at 20-25C
48 hours
10% NBF
4-24 hours
Phosphate Buffered Formalin
4-24 hours
Formol corrosive
3-24 hours
Formol sublimate
3-24 hours
Formol-Mercuric chloride
3-24 hours
Buffered formaldehyde + Osmium tetroxide for small tissue fragments and needle biopsies
2-4 hours
Buffered formaldehyde + Osmium tetroxide for large tissue fragments <4 mm thick
6-8 hours up to 24 hours
Glutaraldehyde
30 minutes to 2 hours
Zenker's fluid
12-24 hours
Zenker-formol/Helly's solution
12-24 hours
Heidenhain's Susa solution
3-12 hours
B-5 fixative
1 hour and 30 minutes to 2 hours
Orth’s fluid
36-72 hours
Bouin’s solution
6-24 hours
Ethyl alcohol
18-24 hours
Carnoy’s fluid
1-3 hours
Newcomer’s fluid at 3C
12-18 hours
Flemming’s solution
24-48 hours
Flemming’s solution without acetic acid
24-48 hours
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