A Level Biology - Manipulating genomes

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Last updated: February 13, 2019

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Defintion	Keyword
A technique that allows genes to be isolated and read	DNA sequencing
Method for introducing a vector with a novel gene into a cell	Electroporation
Small loops of DNA in prokaryotic cells	Plasmids
Enzyme that catalyses the joining of sugar and phosphate groups within DNA	DNA ligase
A biomedical technology in molecular biology that can amplify a short length of DNA to thousands of millions of copies	Polymerase chain reaction

Defintion	Keyword
Gene therapy by inserting functional alleles into body cells	Somatic cell gene therapy
Gene therapy by inserting functional alleles into gametes or zygotes	Germ line gene therapy
Process used to separate proteins or DNA fragments of different sizes	Electrophoresis
In gene technology, anything that can carry/insert DNA into a host organism	Vector
Endonuclease enzymes that cleave DNA molecules at specific recognition sites	Restriction enzymes